Jul 04, 2002 · Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates.Cited by: 1644. Figure 1: RNA interference in adult mice. a, Representative images of light emitted from mice co-transfected with the luciferase plasmid pGL3-control and either no siRNA, luciferase siRNA or.
RNA interference (RNAi) is a form of posttranscriptional gene silencing in which the presence within the cell of double-stranded RNA (dsRNA) leads to the specific degradation of mRNA with a. RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates.
RNAi in adult mice Both siRNAs (RNA duplexes) and shRNAs (expressing constructs in the form of plasmids) can be delivered directly into adult animals via various strategies. In these experiments, the RNAi effect is confined to part of the body, often transient, and will not transmit to the next generation.Cited by: 52. Aug 19, 2005 · RNA interference (RNAi) has revolutionized the study of gene function by target gene knockdown and has the potential to be used therapeutically (reviewed in,). RNAi can be induced in mammalian cells by introduction of small interfering RNA (siRNA),. These are double-stranded RNA molecules 21–23 nucleotides in length and resemble RNA cleavage products resulting from the action Cited by: 29.