Dec 14, 2017 · The UAS/GAL4 system is the most used method in Drosophila melanogaster for directing the expression of a gene of interest to a specific tissue. However, the ability to control the temporal activity of GAL4 with this system is very limited. This study constructed and characterized Tet-off GAL80 transgenes designed to allow temporal control of GAL4 activity in aging adult muscles.Cited by: 2. Oct 25, 2012 · But the GAL4 lines we describe are not individually adequate to provide cell-type specific expression. Even when single 3-kb DNA fragments are used to drive GAL4 expression, a tiny fraction—perhaps less than one in a thousand of patterns is—limited to a single neuronal population in Cited by: 675.
The GAL4/UAS system is commonly used in Drosophila to drive expression of a gene of interest (Brand & Perrimon, 1993). Tissue-specific or ubiquitous enhancers drive expression of GAL4 that results in transcriptional activation from the GAL4 binding sites (UAS) to drive expression of a gene of interest, and is widely used for expression of. Jun 14, 2010 · (2006) reported instances where Split GAL4 intersections showed expression in cells not observed in either parent pattern and we also observed this phenomenon, to varying extents, in many of the Split GAL4 intersections we examined in the adult brain (A. N ern, B. D. P feiffer and G. M. R ubin, unpublished results).Cited by: 804.
Expression patterns of the Janelia GAL4 drivers in the adult brain and ventral nerve cord, in third instar imaginal discs, and in the embryonic CNS can be found at Janelia's FlyLight site (we provide links to expression data for individual GAL4 lines below). The data presented at FlyLight are the work of the Janelia Farm FlyLight Project Team. Producing Lines with Sparse Expression Patterns using the Split-GAL4 Approach. Traditional enhancer trap GAL4 lines (“Generation 0” lines) are expressed, on average, in 300-500 cells of the 30,000-40,000 cells of the central adult fly brain, whereas our Generation 1 lines are expressed more sparsely, in an average of ~100 cells (Jenett).